Chapter 013 Cost
With money as the basis, Li Jinglan soon purchased a large number of mature or semi-mature eels in the coastal areas of southern Fujian Province.
The eels are then transported to Caragua Island via special live seafood transport ships.
at the same time.
Li Qingye also received another message.
Through a satellite phone, Conan, who was out at sea to catch eel fry, reported to him an important piece of information, that is, the number of glass eels in the wild eel breeding area near the Luzon Trench has become extremely scarce.
According to the communication between Conan and more than a dozen colleagues, the number of glass eels at this time is only a few tenths of that in previous years.
Many fishing boats only caught dozens of kilograms of fish fry after several weeks of hard work, while in previous years they could catch several tons of fish fry. This is a cliff-like drop.
Li Qingye naturally understood that the consequences of overfishing were beginning to show, but it was also an opportunity and a huge outlet.
When wild resources are scarce, the price of fish fry will rise rapidly, and capital investment will also be stimulated.
Now he must quickly develop low-cost eel breeding technology to prevent other companies from developing similar technology.
Currently, the research institution that conducts the most in-depth research on artificial breeding of eels is a research institute from Japan. They have already achieved artificial breeding of eels, but the production cost is too high.
The technology developed by Japanese research institutions is to induce mature eels to enter the reproductive state through hormone stimulation and environmental regulation.
Currently, the production cost of an artificially bred eel is as high as US$60,000.
The wholesale price of wild eel fry is US$7,000 to US$12,000 for 5,000 glass eels .
The price difference between the two is too big, and artificial eel fry are not competitive at all.
Li Qingye had read the relevant papers from that research institute and even used hacking methods to turn the other party's network upside down.
Although the research institute concealed some key data, it has recently reduced the production cost to around US$140,000.
However, Li Qingye is not optimistic about them, because the key to this artificial breeding technology lies in special hormones, and the cost of those special hormones is high.
He used data from Japanese researchers to derive a complete set of technologies.
"The key lies in the special sex hormone of eels. Currently, this hormone cannot be synthesized artificially, and the prices of several alternative hormones are not cheap, and they can easily cause developmental abnormalities in eels." Li Qingye sorted out his thoughts.
Soon he began to experiment.
First, through the combined effects of various hormones and the stimulation of simulated environmental stress, the eels are prompted to enter a state of sexual maturity.
They found that special eel sex hormone and extracted it.
By comparing the gene sequences of eels before and after sexual maturity, we found some gene sequences that may be related to sexual maturity.
The next step is very simple, which is to use the CRISPR sequence in combination with the Jianmu factor to inactivate some of the eel genes.
After more than a month of research, he quickly locked down the gene sequence of sexual maturity in eels, cut out these genes, and inserted them into yeast through CRISPR-Cas9.
Soon under environmental pressure, the genetically modified yeast rapidly produced eel sex hormones.
In addition to the equipment, the production cost of this hormone has dropped significantly to around US$5 per gram.
This is still the production cost using laboratory solutions. If an industrialized production solution is adopted, the production cost will continue to decline.
With this special eel sex hormone, he quickly injected it into mature eels.
In the C5 area of the experimental base.
The dozen or so employees responsible for checking the eel breeding tanks were holding pens and recording the conditions of each tank.
Li Qingye and Li Jinglan looked at the situation in each water tank.
"Has it been successful?" Li Jinglan was very excited. Looking at the glass eels in the water tank, he felt as if he saw gold swimming.
Li Qingye said calmly: "Well, the technology is mature. Two mature eels can produce 1 to 3 million eggs. Without interference from other predators, 30% of these eggs can develop into glass eels."
"Boss, we are rich. The price of a portion of eel fry in January this year has reached 30,000 US dollars, and they are still out of stock."
Li Qingye ordered: "Take your people to China, Japan and Southeast Asia as soon as possible and sell the eel fry we have in small batches, but don't reveal that we have artificial breeding technology."
"Work hard, and the company will give you bonus shares in the future. Also, let Kangnan come back! No need to catch fish fry anymore, he will follow you first."
"I understand. I will do my best." Li Jinglan smiled.
Looking at Li Jinglan who was leaving in a hurry, Li Qingye's expression was very calm, but there was something meaningful in his eyes.
Betrayal, this is human nature.
His father believed too much in so-called beliefs, and was stabbed back again and again by his former like-minded partners, and eventually his family was broken up.
"Instead of believing in the faith, it is better to implement the means. Father, I am not naive, and I am not qualified to be naive."
Li Qingye put his hands in his pockets and thought silently . His white coat was pure and cold. He walked in the direction marked Area A.
The entire scientific research base is divided into four levels of areas: A, B, C, and D. Only Li Qingye has the authority to enter areas AB.
Even though he has been researching eel breeding technology for more than a month, in fact, this is just a small project.
It is so difficult to survive in this cruel world and to be a self-reliant person.
Without strength, you can’t protect the Homo sapiens company.
If you don't want to be a dog for others, you can only beat to death those who want to train dogs.
While studying eel breeding technology, he also started a research project on biological weapons.
Enter the core area A.
Looking at the orange liquid in the glass reagent bottle, there are some cells floating in it that are difficult to see with the naked eye. These are Plasmodium, a microorganism that can cause malaria, that has been modified through genetic recombination technology.
Li Qingye carried out a comprehensive transformation on it.
First, it improves its drug tolerance, and no antimalarial drugs on the market have any effect on it;
Second, the reproductive ability was knocked out, making it impossible for the malaria parasite to reproduce naturally.
Third, the dormancy gene sequence of the tardigrade was inserted, giving it an incubation period of about seven years.
Fourth, the insect metamorphosis gene sequences are inserted, and once activated by special pheromones, they will wake up from their dormant state and start to run rampant and cause destruction.
Fifth, the cell symbiosis model, which is to ensure that the malarial parasites will not be digested and decomposed by the human body during their dormancy. The cells that Li Qingye chose for them to parasitize are human brain cells.
At present, the preliminary preparation of [Plasmodium parasite type 1] has been completed.
But Li Qingye still felt that it was not perfect, because [Gu worm-malaria parasite type 1] still had some shortcomings.
For example, it is too obvious that it can only be injected through a syringe. How he deceives others into giving him the injection is a problem.
Another problem is that the special pheromones needed in the activation process also need to be injected, which increases the uncontrollable factors.
He now has to find a way to solve these two problems.
Moreover, if you are prepared for this type of external trigger, you can isolate the trigger of pheromones by wearing protective clothing.
Now it seems that the internally triggered Sanshi Brain Pill seems to make sense.